The Humanized Mouse Core specializes in production of the current standard model in the field — the humanized immune mouse (HIM) model. Other humanized mouse models are available, including the “BLT” mouse, HSC engrafted NSGs, or other custom orders.
Humanized mouse models
Sub-lethally irradiated NOD/SCID/IL2γc null (NSG) mice were surgically implanted with cryopreserved human thymus and tissue fragments under the kidney capsule. CD34+ hematopoietic cells were magnetically separated from umbilical cord blood and injected intravenously. Mice were monitored and the level of human hematopoietic cells in the mice was determined by multicolor flow cytometric analysis.
By 10-12 weeks post-surgery/injection, the humanized mice showed a functional human thymic organoid with typical human anatomical features (Figure A below).
Humanized mice demonstrate systemic repopulation with human lymphohematopoietic cells, including B cells, T cells, monocytes, and dendritic cells (Figure B below). In our functional study, the humanized mice showed responses to a known Th-1 antigen (Tetanus Toxoid) and a Th-17 antigen (Collagen V), indicating that T helper cells educated in the autologous human thymus organoid are functional and provide an adequate level of immunocompetence for study of a wide range of human immunologic processes.