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Authors Brubaker AL, Schneider DF, Palmer JL, Faunce DE, Kovacs EJ
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Journal J. Immunol. Methods Volume: 373 Issue: 1-2 Pages: 161-6
Publish Date 2011 Oct 28
PubMed ID 21889511
PMC ID 3195830
Abstract

Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.

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