|Authors||Brubaker AL, Schneider DF, Palmer JL, Faunce DE, Kovacs EJ|
|Journal||J. Immunol. Methods Volume: 373 Issue: 1-2 Pages: 161-6|
|Publish Date||2011 Oct 28|
Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.
|Full Text||Full text available on PubMed Central|