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Embryonic Stem Cells Pictures


Immunohistochemistry for neurogenin3, a bHLH transcription factor expressed in proliferating islet progenitor cells residing within the pancreatic duct epithelium of an 15.5 day old mouse embryo. Neurogenin 3 positive cells are seen in purple, 40X.	Undifferentiated ES cell colonies (left panel) and embryoid bodies (EBs, middle and right panels) from human and mouse embryos. Right panel is H&E staining of tissue sections. Middle and left panel are low power phase contrast images.

Expression of pancreatic marker proteins in murine differentiated ESCs including PDX1, peptide YY, an early endocrine marker, insulin, glucagon, islet amyloid polypeptide, and pancreatic polypeptide	Human ES cells differentiate into PDX1-expressing cells (red).

Rat insulinoma cells in culture immunostained for pancreas and duodenal homeobox 1 (PDX1, red nuclear staining) and insulin (green cytoplasmic staining).	Insulin and C-peptide expressing cells derived from mouse ESCs.

Pancreas development in human and mouse embryos from gastrulation through post-natal maturation.

PDX1+ cells in mouse ES cell cultures arise in focal clusters (not randomly scattered throughout the monolayer). From within these PDX1+ foci emerge insulin and somatostatin hormone positive cells that also express PDX1 as would be expected for definitive beta and delta cells of the islet, respectively	Hormone positive cells originate from pdx1+ clusters in differentiated mouse ESCs. Nuclear pdx1 immunostaining is seen in red and cells immuostaining for insulin, somatostatin, or glucagon appear green

Rat insulinoma cells stained with anti-pdx1 (red) and anti-insulin (green).	Single human embryoid body (EB) derived from human ESCs.

Spherical embryoid bodies (EBs) derived from murine ES cells, phase contrast imaging.	Tissues characteristic of 3 embryonic germ layers arise in HESC derived teratomas. A) neuroepithelium, B) trabecular bone, C) cartilage, D) skeletal muscle, E) primitive glomeruli, and F) gut-like structure containing mucin-producing columnar epithelium.