The primary objective of the HRC is to provide a broad range of high-quality histology methods and resources to support basic, translational, and clinical science research at our institution and across the greater research community.
Our vision is to offer affordable histological services to investigators that complement, support, and advance the innovative research missions of our clients.
The HRC is here to provide support for your project from start to finish. We are open to all UW investigators and external clients.
Services
Our facility offers a broad range of high-quality histological and immunohistochemical services on a fee-for-service basis. Services include routine histology procedures, staining, custom protocol development, and training/education. We work with animal and human tissue up to BSL 2 and utilize paraffin and frozen techniques. Our routine histology procedures include tissue processing, embedding, frozen and paraffin sectioning, H&E staining, and imaging.
Protocol Development
Our core provides the researcher with a unique opportunity to design and plan a study that optimizes the best tissue processing and staining techniques available. Individual attention to protocol and technique development allows us to offer personalized services for research and development. For researchers, this can look like developing a new antibody titration, collaborating on a dissection technique, creating a new multiplex staining protocol, or a specialized sectioning protocol.
Staining
- H&E
- Special stains:
- We currently offer over 15 different types of special stains, including trichrome, PAS, MSB, ORO, and more, and are always looking for opportunities to expand our catalog.
- IHC:
- Brightfield or immunofluorescent detection.
- We have a catalog of over 70 verified antibodies at our disposal at no additional cost to the researcher.
- Our IHC protocols include multiplex labeling and same-species staining.
Imaging
- The HRC hosts two fluorescent microscopes that are available at an hourly rate for trained users. Please contact the core to set up a training session. Reservations can be made through our iLabs website.
- Nikon E600 Microscope with Fluorescence.
Located in WIMR I, rm 5122. Available 24/7 with afterhours WIMR access.
Microscope is equipped with an Olympus DP73 camera and connected to computer with Cellsens software.
The scope is equipped with the following filters:- V-2E 340-380 excitation with 400nm LP, 435-485 band- DAPI
- B-2E/C 465-495 excitation and 505 LP, 515-555 band for FITC, GFP, AF488
- G-1B 541-551 Excitation and 565 LP and 590 LP – red/orange
- Nikon TiS/L100 Inverted Microscope with Fluorescence.
Located in H4/337, CSC. Available M-F 9:00 – 5:00pm
Microscope is equipped with both a DS-Qi2 monochrome camera for high resolution fluorescent images and a Lumenera Infinity color camera.
The microscope uses the NIS Elements software.
The scope is equiped with the following filters:- DAPI/Hoescht/AF350
- EGFP/FITC/ AF488
- Texas Red/mCherry/ AF594; Cy5
Education/Training
We offer training and education for microscopy and staining. We can work with you one-on-one or in a group as we go over microscopy basics, H&E staining, or IHC fundamentals.
Sample Submission Requirements
- The Histology Resource Core (HRC) DOES NOT accept the following items:
- body fluids of any kind from any species
- whole adult animals
- samples containing prions, or any other biological agents at BSL 3 or higher
Samples that are submitted must either be frozen or placed in fixative.
**Please contact the core if you have any questions about these items**
Acknowledgement
It is your responsibility to cite the Histology Resource Core (HRC) in the Acknowledgments section of your manuscript and notify the Core when manuscripts that used our services are submitted for publication, indicating the journal citation.
“The author(s) thank the University of Wisconsin-Madison, Department of Surgery Histology Resource Core (HRC), Dr. Angela Gibson, MD, PhD, FACS, PI of the HRC, along with certified Histotechnologist Sierra Raglin, HTL (ASCP)CMP and Rebecca Lyons-Oelze, HTL (ASCP).”
ILABS Help
For assistance with your iLabs account or for frequently asked questions please visit the iLab Core Facility Management System. You can also click on the HELP button in the upper right hand corner of the iLabs website, or email their support team at ilab-support@agilent.com.
Pricing
Approvals
IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information.
FAQs
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Where should I drop off/pick up my samples?
Histology samples can be dropped off and picked up from the Histo Core at H4/337 in the Clinical Sciences Center.
What time should I drop off/pick up samples?
Samples may be dropped off or picked up any time between 9am-5pm Monday – Friday. To ensure a smooth drop off or pick up, it is recommended to send an email to the HRC at histocore@surgery.wisc.edu with your estimated time of arrival to ensure that someone is present in the lab when you arrive.
Where can I make a service request?
To make a request, please visit our iLabs website and fill out the form.
What should I do if I need help with iLabs?
If you need help with:
- Creating an iLabs account (as a PI or lab member)
- Requesting a service
- Reserving equipment
- Approving request to join a lab
- And much more
Please visit the UW iLab Resource Center. You can also visit the Agilent iLab help site by clicking on the blue “HELP” button in the upper right hand corner of your iLabs home page. For further help, you can email the UW iLabs support team (support@ilab.wisc.edu) or the Agilent iLabs support team (ilab-support@agilent.com).
Service Requests >>
To place an order, please visit our iLabs website. You will need to create an iLabs account to submit a request.
Contact Us
Principal Investigator: Angela Gibson, MD, PhD, FACS
Core Staff: Sierra Raglin, HTL, Rebecca Lyons-Oelze, HTL
Lab: 608-262-8395 histocore@surgery.wisc.edu
Location: H4/337, Clinical Science Center at University Hospital
Open Hours: 9:00 – 5:00pm
Consultation Hours: 10:00 – 3:00pm
Turn-around time: Typical turn-around time is less than 10 working days. If you need samples quickly, we can accommodate with a rush surcharge.