University of Wisconsin–Madison

Histology Core Service

Histology services are open for business and are currently processing requests.

The UW Department of Surgery Histology Core facility, located at H4/337 in the Clinical Science Center, provides Histology services to clinical and basic science researchers associated with the Department of Surgery. We look forward to extending our services to members of the University of Wisconsin and the local biotechnology community.

The objective of the Core is to provide high-quality stained tissue sections on microscope slides to support clinical and basic science research at our institution.

Our facility offers a broad range of high-quality histological and immunohistochemical services available on a fee-for-service basis to investigators. Services include tissue fixation, processing, embedding, sectioning, staining and coverslipping of paraffin or frozen tissue sections. Staining of tissue sections ranges from routine H&E’s (Hematoxylin-Eosin) Trichrome, Giemsa and PAS. Special stains such as Von Kossa, PicroSirius Red, Elastic and others are available upon request.

Our Core offers immunohistochemical staining with a general selection of antibodies. In addition, our services can complement your research in the creation of protocols that include antibodies for immunofluorescent or immnuperoxidase studies. Double/triple antibody staining services are available upon request. More than 24 optimized mouse and more than 75 optimized human/monkey/dog antibody staining protocols are available.

We also specialize in custom antibody staining optimized to fit your specific needs. Tutorials and equipment use are available.

The Core is managed and operated by a highly experienced Histotechnologist with many years of experience in protocol development and optimization.

The facility is equipped with technological instrumentation including:

  • A Miles Scientific Tissue-Tek VIP Automated Tissue Processor
  • A manual staining series
  • A Fisher HistoCenter embedding station
  • Leitz microtome
  • Leica CM1900 cryostat

IRB or IACUC approval is required for all services requiring the use of human or animal tissue, respectively. Service requests will NOT be processed without the necessary compliance information.

For more information, contact Sierra Raglin at or (608) 262-8395.

To order

To place an order, please visit our iLabs website. You will need to create an iLabs account to submit a request.

  • Antibodies

    Please contact core for current available antibodies.

    Many factors can affect antibody optimization outcomes. If results are not satisfactory, after reasonable attempts are made to optimize your antibody, we will offer other options at our regular prices.

  • Prices
We offer our clients:
  • Low-cost/high-quality preparation
  • Less than a one week turnaround time on routine biopsies
  • Nationwide service with FedEx shipping
  • Computerized monthly billing